Robust Genome Engineering in Primary Human T Cells using CRISPR/Cas9 Ribonucleoproteins

TitleRobust Genome Engineering in Primary Human T Cells using CRISPR/Cas9 Ribonucleoproteins
OwnerUniversity of California

UCSF researchers have developed a powerful Cas9 RNP-based technology that uses purified Cas9 ribonucleoproteins (RNP) for successful and efficient genome editing in primary human CD4+ T cells. Cas9 protein pre-complexed with a single guide RNA (sgRNA) is introduced as an RNP into human T cells by transient electroporation. The active complexes enabled the first successful Cas9-mediated homology directed repair (HDR) in primary human T cells. Cas9 RNPs have allowed generation of ‘knock-in’ primary human T cells with targeted genetic replacement of specific nucleotides, which was previously unattainable.


1) Unprecedented flexibility to ‘knock-out’ and ‘knock-in’ specific genetic elements in engineered T cells for cancer immunotherapy

2) New opportunity for therapeutic gene correction for primary immune deficiencies, treatment of infections and autoimmune diseases

3) Diverse research applications examining the function of coding and non-coding genetic variation in human immune regulation


Stage of Development

Proof of principle

Related Materials


  • Not available at this time

Data Availability

In vitro human data


Applicants:University of California
Access to additional documentation:Please inquire
Case Ref:UC Case 2015-118-0
Application number:please inquire
Support from inventors:Please inquire
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